Effects of Aqueous Extract of Moringa oleifera Seed on the Histology of the Kidney in Wistar Rats

Akukwu Catherine

David Umahi Federal University of Health Sciences, Uburu, Ebonyi State, Nigeria.

Akukwu Darlington

David Umahi Federal University of Health Sciences, Uburu, Ebonyi State, Nigeria.

Aguwa Ugochukwu *

Anatomy Department, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.

Agulanna Ambrose

Anatomy Department, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.

Ukoba Obatavwe

Delta State University Teaching Hospital, Abraka, Nigeria.

Okonkwo Izuchukwu

Nnamdi Azikiwe University Teaching Hospital Nnewi. Nigeria.

Okeke Somadina

Anatomy Department, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.

Nwosu Chioma

Abia State University, Uturu, Nigeria.

Nwankwo Matthew

Anatomy Department, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.

Aguwa Adline

Nnamdi Azikiwe University Teaching Hospital Nnewi. Nigeria.

*Author to whom correspondence should be addressed.


Moringa oleifera, also called the “magic tree”, has a long history of use in the treatment of many diseases including those affecting the liver and kidney. This research work focuses on the effect of aqueous extract of Moringa oleifera seed on the kidney of healthy Wistar rats. Twenty (20) rats weighing between 100 - 150g were divided into four groups (A-D) of five rats each. Group A was control and received food and water. Group B, C and D received 400mg/kg, 800mg/kg and 1600mg/kg of aqueous extract of Moringa Oleifera per body weight respectively. The extract was administered orally for 21 days. On the 22nd day, the rats were sacrificed by cervical dislocation and the kidneys fixed in 10% formalin for histological studies using H&E. Histological observation showed proliferation of the mesangial cells in group B while in groups C and D glomerular sclerosis were observed. This study submits that aqueous extract of Moringa oleifera seed has nephrotoxic tendencies, and this effect was dose dependent.

Keywords: Kidney, moringa oleifera, nephrotoxic, cervical dislocation

How to Cite

Catherine , A., Darlington , A., Ugochukwu , A., Ambrose , A., Obatavwe , U., Izuchukwu , O., Somadina , O., Chioma , N., Matthew , N., & Adline, A. (2023). Effects of Aqueous Extract of Moringa oleifera Seed on the Histology of the Kidney in Wistar Rats. International Journal of Advances in Nephrology Research, 6(1), 80–86. Retrieved from https://journalijanr.com/index.php/IJANR/article/view/46


Download data is not yet available.


Anwar F, Latif S, Asharf M, Gilani AH. Moringa Oleifera-. a food plant with multiple Medicinal Uses. Journal of Botany. 2007;39(5):1443-1453.

Dalen, MB, Pam JS, Izang A, Ekele R. Synergy between Moringa Oleifera seed powder and alum in the purification of domestic water science world journal. 2009;4:125-128 www.sciecnceworldjoumal.org ISSN:1597-6343.

David Shier, Jackie Butler, Ricki Lewis. Hole’s Human Anatomy and Physiology, 12th Edition, McGraw-Hill, New York. 2010;776-778.

Foidl N, Makkar IIPS, Becker K. The potential of Moringa Oleifera for Agricultural and Industrial Uses, in: L.J. Fuglie (Ed.), The Miracle Tree: The Multiple Uses of Moringa Oleifera. CTA, Wageningen, Netherlands. 2001;45-76

Gupta R, Dubey DK, Kannan GM, Flora SJ. concomitant administration of Moringa Oleifera seed powder in the remediation of arsenic - induced oxidative stress in mouse. Ceil Boil International. 2007;44- 56.

Guyton AC, Hall JE. Urine formation by the kidneys: glomerular filtration, renal blood flow, and their control; the body fluids and kidneys. Text Book of Medical Physiology, 111'1 edition. 2006;303–325.

Inderbir Singh. Textbook of human histology with colour atlas, Jaypee Brothers, Medical publishers (P) Ltd New Delhi. 2002;264-270.

Inderbir Singh and GP Pal. Human Embryology, Eighth edition, Rajiv Beri for Macmillan India Ltd, New Delhi. 2007;249-244.

Karahan İ, Ateşşahin A, Yılmaz S, Çeribaşı AO, Sakin F. Protective effect of lycopene on gentamicin-induced oxidative stress and nephrotoxicity in rats. Toxicology. 2005;215(3):198-204.

Kawo AH, Abdullahi BA, Gaiya ZA, Halilu A, Dabai M, Dakare MA. Preliminary Phytochemical Screening, Proximate and Elemental Composition of Moringa oleifera Lam Seed Powder. Bayeio Journal of Pure and Applied Sciences. 2009;2(1):96- 100.

Mangale SM, Chonde SG, Jadhav AS, Raut PD. Study of Moringa Oleifera (Drumstick) seed as natural Absorbent and Antimicrobial agent for River water treatment. Journal of Natural. Production Plant Resource. 2007;87-90.

Moore KL, Dailey AF, Clinically Oriented Anatomy, 5th Edition, Lippincott Williams and Wilkins Publication, UAS, ISSBN 0-7817- 3639-0(2)289-300 5th Moore and Persaud; 2006.

The Developing Human. Clinically Oriented Embryology 6th Edition. WB. Sanders Publication Company. ISSBN 0-58089-2180-0. Pg 245-255

Acquaye D, Smith M, Letchamo W, Simon J. The miracle tree: Center for new use agriculture and natural plant product. Rutgers University, Cook College. 2000;65-78

Le Strange R, Angus, Robertson. A history of herbal plants.1977;24

Poon MK, Chiu PY, Leung HY, Siu AH, Ko KM. A yang invigorating Chinese herbal formula protects against gentamicin- induced nephrotoxicity in rats. Department of Biochemistry, Hong Kong University of Science and Technology, clear water bay, Hong Kong SAR, China; 2007.

Romanes GJ, Cunningham’s Manual of Practical Anatomy: 15th Edition, volume 2 Oxford University press inc; New York. Pg 89 – 97.

William F. Ganong; Review of Medical Physiology, 21st Edition, Lange Medical Books, McGraw - hill publications, USA, 2003 ISSBN -0-07-121765 - 7 (25) 502 - 508. Pg 250 - 265

Young B, Health JW, Stevens A, Lowe SJ, Deakin JP. Kidney in the Urinary System. Wheater’s Functional Histology. A Text and Colour Atlas, 4lh Edition. 2000;303–320.

Ajibade TO, Olayemi FO, Arowolo ROA. The Haematological and Biochemical effects of methanolic extract of the seeds of Moringa Oleifera in rats. Department of Veterinary Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria. Journal of Medicinal Plants Research. 2012;6(4):615-621.

Sinha M, Manna P, Sil PC. Amelioration of galactosamine — induced nephrotoxicity by a protein isolated from the leayes of the herb Cajanumus Indicus L. department of Chemistry, Bose institute, Kolkata. 2007: 34-40.