Effect of Methanolic Stem Bark Extract of Acacia nilotica (Bagaruwa) on Some Renal Function Parameters in Alloxan Induced Diabetic Albino Rats

Kabiru Sirajo *

Department of Biochemistry, Sokoto State University, Sokoto, Nigeria.

Zulkallaini Shehu

Department of Biochemistry, Sokoto State University, Sokoto, Nigeria.

Ibrahim Mas’ud

Department of Environmental Science, Islamic University in Uganda, Uganda.

Musa Muhammad Dan-Ige

Department of Biochemistry, Sokoto State University, Sokoto, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Background: Diabetes Mellitus (DM) is a complex metabolic disorder characterize by high blood sugar which gives rise to many complications including diabetic nephropathy. While there were enormous reports on the uses and efficacy of various medicinal plants, very few information are available on their safety and side effects while been used to treat certain ailment.

Aim: The aim of this study is to determine the effect of methanolic stem bark extract of Acacia nilotica on some renal function parameters in alloxan induced diabetic albino Rats.

Methodology: Thirty (30) young albino rats were grouped into six (6), comprising of five (5) rats per group. Diabetes was induced by single intraperitoneal injection of freshly prepared alloxan monohydrate and blood glucose level was determined forty eight hours (48 hrs) after injection. After induction of diabetes, Metformin and selected doses of Acacia nolitica extract were orally administered for 4 weeks after which serum biochemical markers were determined.

Results: Acacia nilotica and metformin decreased fasting blood glucose levels compared to diabetic control Group. No significant changes were observed in electrolytes level with the exception of urea and creatinine that significantly (P˂0.05) decreased at 1200 mg/kg and 600 mg/kg respectively compared to control.

Conclusion: The present study revealed that, the plant extract possessed hypoglycemic effect and that it have nephrotoxic protection effect.

Keywords: Acacia nolitica, albino rats, diabetic mellitus, electrolytes, fasting blood sugar, metformin, stem bark


How to Cite

Sirajo, K., Shehu, Z., Mas’ud, I., & Dan-Ige, M. M. (2022). Effect of Methanolic Stem Bark Extract of Acacia nilotica (Bagaruwa) on Some Renal Function Parameters in Alloxan Induced Diabetic Albino Rats. International Journal of Advances in Nephrology Research, 5(1), 98–103. Retrieved from https://journalijanr.com/index.php/IJANR/article/view/35

Downloads

Download data is not yet available.

References

Shubham K, Anu M, Dinesh B, Amit M. Herbal medicines for diabetes management and its secondary complications. Current Diabetes Reviews. 2021;17:437-56.

Kajal A, Singh R. Coriandrum sativum seeds extract mitigate progression of diabetic nephropathy in experimental rats via AGEs inhibition. Plos One. 2019;14: e0213147.

Molitoris BA. Acute kidney injury. In Goldman L, Ausiello D, editors. Cecil Medicine. 23rd ed. ed: Philadelphia, Pa: Saunders Elsevier; 2017.

Singh R, Kaur N, Kishore L, Kumar GK. Management of diabetic complications: A chemical constituents based approach. Journal of Ethnopharmacology. 2013;150: 51-70.

Singh R, Kishore L, Kaur N. Diabetic peripheral neuropathy: Current perspective and future directions. Pharmacological Research. 2014;21-35.

Young EC, Soo KA, Won TL, Jong EL, Seung HP, Bang BY et al. Soybeans ameliorate diabetic nephropathy in rats. Advances in Accounting Publishing. 2010; 4:433-40.

Gupta P, Sharma P, Shanno K, Jain V, Pareek A, Agarwal P et al. Nephroprotective role of alcoholic extract of Pterocarpus marsupium heartwood against experimentally induced diabetic nephropathy. Journal of Pharmacy & Pharmacology Reseaserch. 2016;4:174-86.

Ekakitie L, Ajiboye BO, Oyinloye BE, Owero-Ozeze OS, Onikanni SA, Ojo OA. Attenuation of diabetic nephropathy in alloxan-induced diabetic rats by Solanum macrocarpon Linn aqueous leaves extract. Comparative Clinical Pathology. 2021; 30:173-9.

Hsu J, Wu C, Hung C, Wang C, Huang H. Myrciaria cauliflora extract improves diabetic nephropathy via suppression of oxidative stress and inflammation in streptozotocin-nicotinamide mice. Journal of Food and Drug Analysis; 2016.

Sefi M, Fetoui H, Soudani N, Chtourou Y, Makni M, Zeghal N. Artemisia campestris leaf extract alleviates early diabetic nephropathy in rats by inhibiting protein oxidation and nitric oxide end products. Pathology – Research and Practice. 2012; 208:157-62.

Tripathi P, Singh A. Acacia nilotica, Albizia saman, Azadirachta indica: Ethanobotany and medicinal uses. Modern Research in Botany. 2020;1:96-103.

Aremu O, Olayemi O, Ajala T, Isimi Y, Oladosu P, Ekere K et al. Antibacterial evaluation of Acacia nilotica Lam (Mimosaceae) seed extract in dermatological preparations. Journal Research in Pharmacy. 2020;24:170-81.

Bachaya HA, Iqbal Z, Khan MN, Sindhu ZU, Jabbar A. Anthelmintic activity of Ziziphus nummularia (bark) and Acacia nilotica (fruit) against Trichostrongylid nematodes of sheep. Journal of Ethnopharmacology. 2009;123:325-9.

Dongmo AB, Nguelefack T, Lacaille-Dubois MA. Antinociceptive and anti-inflammatory activities of Acacia pennata wild (Mimosaceae). Journal of Ethnopharmacology. 2005;98:201-6.

Goronyo JI, Ibrahim YKE, Tytler BA, Hussaini M. In vivo antitrypanosomal activities of Acacia nilotica stem bark methanol extract in Wistar rats infected with Trypanosoma brucei brucei. AROC in Natural Products Research. 2022;2:21-7.

Jodi SM, Omoniyi S, Ungo-kore HY, Ngwai YB. Antifungal activities of honey and Acacia nilotica extracts. FUDMA Journal of Sciences. 2020;4:779-83.

Lam SK, Ng TB. A dimeric high-molecular-weight chymotrypsin inhibitor with antitumor and HIV-1 reverse transcriptase inhibitory activities from seeds of Acacia confusa. Phytomedicine. 2010;7:621-5.

Niyodusenga A, Bukachi FO, Kiama TN. Cholesterol lowering Effects of Acacia nilotica subalata in normal and type 2 diabetic male rats. Rwanda Medical Journal. 2019;76:1-5.

Salissou MTM, Tanko Y, Yaro AH, Magaji RA. Analgesic and Anti-inflammatory activies of N-buthanol portion of Acacia nilotica pods in mice and wistar rats. int J Sci Res Man. 2022;10:2321-3418.

Shoge M, Amusan T. Phytochemical, Antidiarrhoeal activity, Isolation and Characterisation of 11-Octadecenoic Acid, Methyl ester Isolated from the seeds of Acacia nilotica Linn. Journal of Biotechnology and Immunology. 2020;2.

Abdulhamid A, Ismail AM, Sani I, Sulaiman A, Kabir A. Acute and sub-chronic toxicity evaluation of crude methanolic leaves extract of Acacia nilotica (Linn.). Res J Med Plants. 2019;13:109-18.

Adebayo JO, Yakubu MT, Egwim CE, Owoyele BV, Enaibe BU. Effect of ethanolic extract of Khaya senegalensis on some biochemical parameters of rat kidney. Journal of Ethnopharmacology. 2003;88:69-72.

Yakubu MT, Bilbis LS, Lawal M, Akanji MA. Evaluation of selected parameters of rat liver and kidney function following repeated administration of yohimbine. Biokemistri. 2003;15:50-6.

Tanko Y, Ibrahim RA, Muhammad A, Eze ED, Jimoh A, Mohammed KA et al. Evaluation of the effect of crude extract and aqueous fraction of Acacia nilotica leaves on haematological indices and serum electrolytes levels on diabetic wistar rats. Journal of Advances in Biology & Biotechnology. 2015;4:1-7.

Ikpi DE, Obembe AO, Nku CO. Aqueous leaf extract of rothmannia longiflora Improves basal metabolic rate and electrolyte parameters in alloxan induced diabetic rats. Nigerian Journal of Physiological Sciences. 2009; 24:67-71.